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Image Search Results
Journal: Antioxidants & Redox Signaling
Article Title: Salusin-β Promotes Vascular Calcification via Nicotinamide Adenine Dinucleotide Phosphate/Reactive Oxygen Species-Mediated Klotho Downregulation
doi: 10.1089/ars.2019.7723
Figure Lengend Snippet: The level of salusin-β in calcifying VSMCs and the aortas of rats. (A) Protein levels of salusin-β determined by ELISA, (B) mRNA levels of salusin-β, and (C, D) salusin-β protein expression in VSMCs stimulated by calcification medium at days 0, 3, 7, 10, and 14. Full blot is shown in Supplementary Figure S16. (E) Immunofluorescence staining of salusin-β in VSMCs at day 14 of stimulation. (F) Salusin-β levels in plasma, (G) mRNA level of salusin-β, and (H) protein level of salusin-β in the aortas of VC rats. (I) Immunohistochemistry staining of salusin-β in the aortas of VC rats. (J) Immunofluorescence double staining showed smooth muscle marker α-SMA (green) and salusin-β (red). Nuclei were stained by DAPI (blue). (K) Immunofluorescence double staining showed endothelial cell marker CD31 (green) and salusin-β (red). Nuclei were stained by DAPI (blue). Values are expressed as mean ± SE. *p < 0.05 versus 0 day, †p < 0.05 versus Control. n = 4–6 for each group. α-SMA, alpha smooth muscle actin; ELISA, enzyme-linked immunosorbent assay; mRNA, messenger RNA; SE, standard error; VC, vascular calcification; VSMCs, vascular smooth muscle cells. Color images are available online.
Article Snippet: Enzyme-linked immunosorbent assay The levels of salusin-β were quantified using commercially available rat enzyme-linked
Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Immunofluorescence, Staining, Immunohistochemistry, Double Staining, Marker