Journal: International Journal of Molecular Medicine

Article Title: Connexin 43 in splenic lymphocytes is involved in the regulation of CD4 + CD25 + T lymphocyte proliferation and cytokine production in hypertensive inflammation

doi: 10.3892/ijmm.2017.3201

Figure Lengend Snippet: Determination of the concentration of pro-inflammatory cytokines in the serum of spontaneously hypertensive rats (SHRs) and Wistar-Kyoto (WKY) rats by ELISA. (A) Interleukin (IL)-2. (B) IL-6. Results represent the means ± SEM. * p<0.05 compared with the WKY rats (n=15).

Article Snippet: Rat IL-2 and rat IL-6 enzyme-linked immunosorbent assay (ELISA) kits were provided by MultiSciences Biotech Co., Ltd. (Hangzhou, China).

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: International Journal of Molecular Medicine

Article Title: Connexin 43 in splenic lymphocytes is involved in the regulation of CD4 + CD25 + T lymphocyte proliferation and cytokine production in hypertensive inflammation

doi: 10.3892/ijmm.2017.3201

Figure Lengend Snippet: Comparisons of the proliferation and inhibition rate of lymphocytes expressing interleukin-2 (IL-2) and IL-6 between spontaneously hypertensive rats (SHRs) and WKY rats. (A) Proliferation rate. (B) Inhibition rate. * p<0.05 and ** p<0.01 compared with the WKY rats.

Article Snippet: Rat IL-2 and rat IL-6 enzyme-linked immunosorbent assay (ELISA) kits were provided by MultiSciences Biotech Co., Ltd. (Hangzhou, China).

Techniques: Inhibition, Expressing

Journal: Drug Design, Development and Therapy

Article Title: Sitagliptin attenuates cardiomyopathy by modulating the JAK/STAT signaling pathway in experimental diabetic rats

doi: 10.2147/DDDT.S109287

Figure Lengend Snippet: Effect of sitagliptin on ( A ) troponin I level and ( B ) CK-MB activity. Notes: Data are mean ± SEM (N=8). * P <0.05 and ** P <0.01. Abbreviations: CK-MB, creatine kinase MB; SEM, standard error of the mean.

Article Snippet: Serum levels of troponin I and activity of creatine kinase MB (CK-MB) were measured using specific rat enzyme-linked immunosorbent assay kits purchased from EIAab (Wuhan, People’s Republic of China) according to the manufacturer’s instructions.

Techniques: Activity Assay

Journal: Scientific Reports

Article Title: Sacubitril/valsartan (LCZ696) ameliorates hyperthyroid-induced cardiac hypertrophy in male rats through modulation of miR-377, let-7 b, autophagy, and fibrotic signaling pathways

doi: 10.1038/s41598-022-18860-y

Figure Lengend Snippet: The effect of the LCZ696 and VAL administration on the hemodynamic parameters, cardiac hypertrophy markers, serum thyroid hormonal level ( A – J ). ( A ) serum NT-proBNP level (Pg/mL). ( B ) serum FGF23 level (ng/mL), ( C ) serum FT4 level (Pmol/L), ( D ) serumFT3 level (Pmol/L), ( E ) body weight (g), ( F ) heart weight (g), ( G ) heart/body weight ratio, ( H ) heart rate (HR) bpm, ( I ) systolic blood pressure (SBP) mmHg, ( J ) left ventricular systolic pressure (LVSP) mmHg, and ( K ) heart weight/tibia ratio. Values are mean of 6 rats per group ± S.E.M * p < 0.05; ** p < 0.01; *** p < 0.001. Group means were compared by one-way analysis of variance (ANOVA) followed by Tukey post hoc tests for multiple comparisons.

Article Snippet: Free thyroxine FT4, triiodothyronine FT3, pro-B-type natriuretic peptide (NT-proBNP), and FGF-23 were measured in serum using rat enzyme-linked immunosorbent assay kits (thyroxine FT4: CUSABIO, Wuhan, China, code CSB-E05079r; triiodothyronine FT3: NT-proBNP: MyBioSource, San Diego, CA, USA, Cat# MBS704791; FGF23: MyBioSource, San Diego, CA, USA, Cat# MBS2515950) according to the respective manufacturer's instructions.

Techniques:

Journal: Antioxidants & Redox Signaling

Article Title: Salusin-β Promotes Vascular Calcification via Nicotinamide Adenine Dinucleotide Phosphate/Reactive Oxygen Species-Mediated Klotho Downregulation

doi: 10.1089/ars.2019.7723

Figure Lengend Snippet: The level of salusin-β in calcifying VSMCs and the aortas of rats. (A) Protein levels of salusin-β determined by ELISA, (B) mRNA levels of salusin-β, and (C, D) salusin-β protein expression in VSMCs stimulated by calcification medium at days 0, 3, 7, 10, and 14. Full blot is shown in Supplementary Figure S16. (E) Immunofluorescence staining of salusin-β in VSMCs at day 14 of stimulation. (F) Salusin-β levels in plasma, (G) mRNA level of salusin-β, and (H) protein level of salusin-β in the aortas of VC rats. (I) Immunohistochemistry staining of salusin-β in the aortas of VC rats. (J) Immunofluorescence double staining showed smooth muscle marker α-SMA (green) and salusin-β (red). Nuclei were stained by DAPI (blue). (K) Immunofluorescence double staining showed endothelial cell marker CD31 (green) and salusin-β (red). Nuclei were stained by DAPI (blue). Values are expressed as mean ± SE. *p < 0.05 versus 0 day, †p < 0.05 versus Control. n = 4–6 for each group. α-SMA, alpha smooth muscle actin; ELISA, enzyme-linked immunosorbent assay; mRNA, messenger RNA; SE, standard error; VC, vascular calcification; VSMCs, vascular smooth muscle cells. Color images are available online.

Article Snippet: The levels of salusin-β were quantified using commercially available rat enzyme-linked immunosorbent assay (ELISA) kits (USCN Life Science, Houston, TX) according to the manufacturer's instructions ( 49 ).

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Immunofluorescence, Staining, Immunohistochemistry, Double Staining, Marker